| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Kings Hammer 2012 MSC Strikers JDL 2 |
0515-01DB0U-0150 | Blake Moore |
| A2 | Chattanooga FC Academy Chattanooga FC Academy - 2012B Navy |
0316-029B0U-7837 | Stephen Hirayama |
| A3 | FC Alliance ChallengeTeams FC Alliance 2012B West 2 |
0204-02SB0U-0004 | Caleb Lucas |
| A4 | Heroes FA - 01 Heroes FA - 0 - B10U |
0445-01SJB0U-0002 | Jody Orellana |
| A5 | Tennessee Soccer Club TSC Williamson 2012 Boys Academy Elite White - JDL |
0522-01AB0U-2083 | Michael Otto |
| A6 | Tennessee Soccer Club TSC Williamson 2012 Boys Academy Select Blue - JDL |
0522-01AB0U-2084 | Michael Otto |
| A7 | Tennessee United Soccer Club TUSC 2012 Boys Academy Blue |
0424-01AB0U-9059 | Nathanael Hubert |
| A8 | Wilson United Soccer Club Wilson United - B10U Black |
0406-01AB0U-0017 | Graham Mattes |
| A9 | Clarksville Soccer Club Clarksville SC - B2012 Maroon |
0422-01AB0U-0015 | Michael Massie |
| A10 | FC Alliance ChallengeTeams FC Alliance 2012B West |
0204-02SB0U-0005 | Filip Leander |